1 Chromatography is useful in forensic science for: A Burning samples B Identifying substances C Mixing samples D Neutralization
2 Which chromatography is faster? A Paper chromatography B Thin-layer chromatography C Distillation D Filtration
3 Why is the baseline drawn in pencil? A Ink dissolves in solvent B Pencil dissolves C Ink is colourless D Pencil is darker
5 Which application uses chromatography in medicine? A Identifying drugs B Measuring pulse C Blood pressure check D X-ray
6 Which paper is commonly used in paper chromatography? A Filter paper B Newspaper C Plastic sheet D Carbon paper
7 Which factor does NOT affect Rf value? A Type of solvent B Stationary phase C Temperature D Shape of container
8 What is chromatography mainly used for? A Mixing substances B Separating components of a mixture C Heating substances D Measuring temperature
9 Which factor affects separation in chromatography? A Boiling point B Affinity for stationary phase C Density only D Mass of solute
10 Which phase remains fixed in chromatography? A Mobile phase B Liquid phase C Stationary phase D Gas phase
11 Which is an application of chromatography? A Detecting food dyes B Measuring mass C Heating chemicals D Cooling solutions
12 If two substances have same Rf value, it means they are: A Definitely identical B Completely different C Likely to be similar D Unstable
13 Which substance will have Rf = 0? A One that moves with solvent B One that does not move C One that moves halfway D One that dissolves fully
15 Which ink component travels the farthest? A Most adsorbed B Least soluble C Most soluble in solvent D Darkest colour
16 Chromatography separates substances based on: A Electrical conductivity B Adsorption and solubility C Magnetism D Radioactivity
18 Which phase moves during chromatography? A Stationary phase B Solid phase C Mobile phase D Adsorbed phase
20 Which component moves fastest in chromatography? A Most soluble in stationary phase B Least soluble in mobile phase C Most soluble in mobile phase D Heaviest component
22 Rf value is calculated by: A Distance moved by solvent / distance moved by solute B Distance moved by solute / distance moved by solvent C Time / distance D Mass / volume
24 What does a single spot on chromatogram indicate? A Mixture B Impure substance C Pure substance D Solvent only
25 In thin-layer chromatography, the stationary phase is usually: A Paper B Water C Silica gel or alumina D Alcohol
26 What acts as stationary phase in paper chromatography? A Water trapped in paper fibers B Ink C Solvent D Air
27 Which colour component usually moves slowest? A Most soluble B Least adsorbed C Strongly adsorbed D Lightest
28 In a chromatogram, separated substances appear as: A Lines only B Spots or bands C Bubbles D Crystals
29 Which principle is the basis of chromatography? A Difference in solubility B Difference in melting point C Difference in colour only D Difference in density